People

Vladi Karniychuk

DVM, PhD

Assistant Professor

Contact

Karniychuk.1@osu.edu (614) 247-9884 Goss Laboratory
1925 Coffey Road
Columbus, OH 43210
Map Link

Department

Veterinary Biosciences

Professional Training and experience

  • Assistant Professor: Department of Veterinary Biosciences, The Ohio State University
  • Adjunct Professor: The School of Public Health and Veterinary Microbiology Department, University of Saskatchewan, Canada
  • Principal investigator: Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Canada
  • Postdoctoral Fellow: McMaster University, Hamilton and Toronto General Research Institute, University Health Network, Canada
  • Ph.D.: Virology, Ghent University, Belgium
  • Researcher (trainee): Virology and Epidemiology, Institute of Experimental Veterinary Sciences, Belarus
  • Doctor of Veterinary Medicine (DVM): Vitebsk State Academy of Veterinary Medicine, Belarus

Research Interests

  • Virus genome biology
  • Interactions of wild-type and dinucleotide-recoded viruses with host proteins
  • Development live vaccines
  • Development oncolytic viruses
  • Flavivirus pathogenesis
  • Non-vector flavivirus transmission
  • Virus interactions with pregnant host and fetus

Selected Awards and Honors

  • 2021    The Bhagirath Singh Early Career Prize in Infection and Immunity. Highest-ranked new investigator in the field of Infection and Immunity in the Canadian Institutes of Health Research Project Grant competition.
  • 2017    Santé Excellence Award, Top Establishment Grant, Biomedical Research, Saskatchewan Health Research Foundation, Canada.
  • 2015    Canadian Institutes of Health Research Postdoctoral Fellowship.
  • 2014    Toronto General Research Institute Postdoctoral Fellowship.
  • 2011    BOF Ph.D. Grant, Special Research Fund (BOF), Ghent University, Belgium.
  • 2009    BOF Ph.D. Grant, Special Research Fund (BOF), Ghent University, Belgium.
  • 2007    INTAS Young Scientist Fellowship, EU Commission.

 

 

 

Publications

  • Udenze D, Trus I, Lipsit S, Napper S, Karniychuk U. Offspring affected with in utero Zika virus infection retain molecular footprints in the bone marrow and blood cells. Emerg Microbes Infect. 2023 Dec;12(1):2147021. doi: 10.1080/22221751.2022.2147021. PubMed PMID: 36369716; PubMed Central PMCID: PMC9869997.
  • Udenze D, Trus I, Berube N, Karniychuk U. CpG content in the Zika virus genome affects infection phenotypes in the adult brain and fetal lymph nodes. Front Immunol. 2022;13:943481. doi: 10.3389/fimmu.2022.943481. eCollection 2022. PubMed PMID: 35983032; PubMed Central PMCID: PMC9379343.
  • Chapagain S, Pal Singh P, Le K, Safronetz D, Wood H, Karniychuk U. Japanese encephalitis virus persists in the human reproductive epithelium and porcine reproductive tissues. PLoS Negl Trop Dis. 2022 Jul;16(7):e0010656. doi: 10.1371/journal.pntd.0010656. eCollection 2022 Jul. PubMed PMID: 35905074; PubMed Central PMCID: PMC9337681.
  • Trus I, Udenze D, Karniychuk U. Generation of CpG-Recoded Zika Virus Vaccine Candidates. Methods Mol Biol. 2022;2410:289-302. doi: 10.1007/978-1-0716-1884-4_14. PubMed PMID: 34914053.
  • Udenze D, Trus I, Munyanduki H, Berube N, Karniychuk U. The Isolated in Utero Environment Is Conducive to the Emergence of RNA and DNA Virus Variants. Viruses. 2021 Sep 14;13(9). doi: 10.3390/v13091827. PubMed PMID: 34578408; PubMed Central PMCID: PMC8473323.
  • Trus I, Berube N, Jiang P, Rak J, Gerdts V, Karniychuk U. Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells. Viruses. 2020 May 25;12(5). doi: 10.3390/v12050579. PubMed PMID: 32466170; PubMed Central PMCID: PMC7290362.
  • Ambagala A, Truong T, Cottam-Birt C, Berhane Y, Gerdts V, Karniychuk U, Safronetz D, Babiuk S. Susceptibility of Chicken Embryos, Sheep, Cattle, Pigs, and Chickens to Zika Virus Infection. Front Vet Sci. 2020;7:23. doi: 10.3389/fvets.2020.00023. eCollection 2020. PubMed PMID: 32118055; PubMed Central PMCID: PMC7012786.
  • Trus I, Udenze D, Berube N, Wheler C, Martel MJ, Gerdts V, Karniychuk U. CpG-Recoding in Zika Virus Genome Causes Host-Age-Dependent Attenuation of Infection With Protection Against Lethal Heterologous Challenge in Mice. Front Immunol. 2019;10:3077. doi: 10.3389/fimmu.2019.03077. eCollection 2019. PubMed PMID: 32038625; PubMed Central PMCID: PMC6993062.
  • Trus I, Walker S, Fuchs M, Udenze D, Gerdts V, Karniychuk U. A Porcine Model of Zika Virus Infection to Profile the In Utero Interferon Alpha Response. Methods Mol Biol. 2020;2142:181-195. doi: 10.1007/978-1-0716-0581-3_15. PubMed PMID: 32367368.
  • Trus I, Udenze D, Cox B, Berube N, Nordquist RE, van der Staay FJ, Huang Y, Kobinger G, Safronetz D, Gerdts V, Karniychuk U. Subclinical in utero Zika virus infection is associated with interferon alpha sequelae and sex-specific molecular brain pathology in asymptomatic porcine offspring. PLoS Pathog. 2019 Nov;15(11):e1008038. doi: 10.1371/journal.ppat.1008038. eCollection 2019 Nov. PubMed PMID: 31725819; PubMed Central PMCID: PMC6855438.
  • Udenze D, Trus I, Berube N, Gerdts V, Karniychuk U. The African strain of Zika virus causes more severe in utero infection than Asian strain in a porcine fetal transmission model. Emerg Microbes Infect. 2019;8(1):1098-1107. doi: 10.1080/22221751.2019.1644967. PubMed PMID: 31340725; PubMed Central PMCID: PMC6711198.
  • Trus I, Darbellay J, Huang Y, Gilmour M, Safronetz D, Gerdts V, Karniychuk U. Persistent Zika virus infection in porcine conceptuses is associated with elevated in utero cortisol levels. Virulence. 2018;9(1):1338-1343. doi: 10.1080/21505594.2018.1504558. PubMed PMID: 30058440; PubMed Central PMCID: PMC7000198.
  • Nguyen PV, Wessels JM, Mueller K, Vahedi F, Anipindi V, Verschoor CP, Chew M, Deshiere A, Karniychuk U, Mazzulli T, Tremblay MJ, Ashkar AA, Kaushic C. Frequency of Human CD45+ Target Cells is a Key Determinant of Intravaginal HIV-1 Infection in Humanized Mice. Sci Rep. 2017 Nov 10;7(1):15263. doi: 10.1038/s41598-017-15630-z. PubMed PMID: 29127409; PubMed Central PMCID: PMC5681573.
  • Darbellay J, Cox B, Lai K, Delgado-Ortega M, Wheler C, Wilson D, Walker S, Starrak G, Hockley D, Huang Y, Mutwiri G, Potter A, Gilmour M, Safronetz D, Gerdts V, Karniychuk U. Corrigendum to "Zika Virus Causes Persistent Infection in Porcine Conceptuses and May Impair Health in Offspring". EBioMedicine. 2017 Nov;25:187. doi: 10.1016/j.ebiom.2017.10.021. Epub 2017 Oct 21. PubMed PMID: 29156210; PubMed Central PMCID: PMC5704060.
  • Darbellay J, Cox B, Lai K, Delgado-Ortega M, Wheler C, Wilson D, Walker S, Starrak G, Hockley D, Huang Y, Mutwiri G, Potter A, Gilmour M, Safronetz D, Gerdts V, Karniychuk U. Zika Virus Causes Persistent Infection in Porcine Conceptuses and may Impair Health in Offspring. EBioMedicine. 2017 Nov;25:73-86. doi: 10.1016/j.ebiom.2017.09.021. Epub 2017 Sep 21. PubMed PMID: 29097124; PubMed Central PMCID: PMC5704061.
  • Darbellay J, Lai K, Babiuk S, Berhane Y, Ambagala A, Wheler C, Wilson D, Walker S, Potter A, Gilmour M, Safronetz D, Gerdts V, Karniychuk U. Neonatal pigs are susceptible to experimental Zika virus infection. Emerg Microbes Infect. 2017 Feb 15;6(2):e6. doi: 10.1038/emi.2016.133. PubMed PMID: 28196970; PubMed Central PMCID: PMC5322322.
  • Karniychuk UU. Analysis of the synonymous codon usage bias in recently emerged enterovirus D68 strains. Virus Res. 2016 Sep 2;223:73-9. doi: 10.1016/j.virusres.2016.06.015. Epub 2016 Jun 27. PubMed PMID: 27364082.
  • Saha D, Karniychuk UU, Huang L, Geldhof M, Vanhee M, Lefebvre DJ, Meerts P, Ducatelle R, Doorsselaere JV, Nauwynck HJ. Unusual outcome of in utero infection and subsequent postnatal super-infection with different PCV2b strains. Virol Sin. 2014 Jun;29(3):176-82. doi: 10.1007/s12250-014-3431-0. Epub 2014 Jun 17. PubMed PMID: 24950783; PubMed Central PMCID: PMC8206427.
  • Geldhof MF, Van Breedam W, De Jong E, Lopez Rodriguez A, Karniychuk UU, Vanhee M, Van Doorsselaere J, Maes D, Nauwynck HJ. Antibody response and maternal immunity upon boosting PRRSV-immune sows with experimental farm-specific and commercial PRRSV vaccines. Vet Microbiol. 2013 Dec 27;167(3-4):260-71. doi: 10.1016/j.vetmic.2013.08.017. Epub 2013 Aug 28. PubMed PMID: 24041768.
  • Karniychuk UU, Nauwynck HJ. Pathogenesis and prevention of placental and transplacental porcine reproductive and respiratory syndrome virus infection. Vet Res. 2013 Oct 7;44(1):95. doi: 10.1186/1297-9716-44-95. Review. PubMed PMID: 24099529; PubMed Central PMCID: PMC4021427.
  • Karniychuk UU, De Spiegelaere W, Nauwynck HJ. Porcine reproductive and respiratory syndrome virus infection is associated with an increased number of Sn-positive and CD8-positive cells in the maternal-fetal interface. Virus Res. 2013 Sep;176(1-2):285-91. doi: 10.1016/j.virusres.2013.05.005. Epub 2013 May 23. PubMed PMID: 23707347.
  • Karniychuk UU, Saha D, Vanhee M, Geldhof M, Cornillie P, Caij AB, De Regge N, Nauwynck HJ. Impact of a novel inactivated PRRS virus vaccine on virus replication and virus-induced pathology in fetal implantation sites and fetuses upon challenge. Theriogenology. 2012 Oct 15;78(7):1527-37. doi: 10.1016/j.theriogenology.2012.06.015. PubMed PMID: 22980086.
  • Geldhof MF, Vanhee M, Van Breedam W, Van Doorsselaere J, Karniychuk UU, Nauwynck HJ. Comparison of the efficacy of autogenous inactivated Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) vaccines with that of commercial vaccines against homologous and heterologous challenges. BMC Vet Res. 2012 Oct 3;8:182. doi: 10.1186/1746-6148-8-182. PubMed PMID: 23031319; PubMed Central PMCID: PMC3514241.
  • Karniychuk UU, Van Breedam W, Van Roy N, Rogel-Gaillard C, Nauwynck HJ. Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV infection. Vet Res. 2012 Mar 16;43(1):19. doi: 10.1186/1297-9716-43-19. PubMed PMID: 22423651; PubMed Central PMCID: PMC3368719.
  • select
  • Nauwynck HJ, Van Gorp H, Vanhee M, Karniychuk U, Geldhof M, Cao A, Verbeeck M, Van Breedam W. Micro-dissecting the pathogenesis and immune response of PRRSV infection paves the way for more efficient PRRSV vaccines. Transbound Emerg Dis. 2012 Mar;59 Suppl 1:50-4. doi: 10.1111/j.1865-1682.2011.01292.x. Epub 2012 Jan 9. Review. PubMed PMID: 22225918.
  • Van Doorsselaere J, Brar MS, Shi M, Karniychuk U, Leung FC, Nauwynck HJ. Complete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virus. Virus Genes. 2012 Feb;44(1):51-4. doi: 10.1007/s11262-011-0665-x. Epub 2011 Sep 27. PubMed PMID: 21948018.
  • Karniychuk UU, Saha D, Geldhof M, Vanhee M, Cornillie P, Van den Broeck W, Nauwynck HJ. Porcine reproductive and respiratory syndrome virus (PRRSV) causes apoptosis during its replication in fetal implantation sites. Microb Pathog. 2011 Sep;51(3):194-202. doi: 10.1016/j.micpath.2011.04.001. Epub 2011 Apr 13. PubMed PMID: 21511026.
  • Van Breedam W, Costers S, Vanhee M, Gagnon CA, Rodríguez-Gómez IM, Geldhof M, Verbeeck M, Van Doorsselaere J, Karniychuk U, Nauwynck HJ. Porcine reproductive and respiratory syndrome virus (PRRSV)-specific mAbs: supporting diagnostics and providing new insights into the antigenic properties of the virus. Vet Immunol Immunopathol. 2011 Jun 15;141(3-4):246-57. doi: 10.1016/j.vetimm.2011.03.008. Epub 2011 Mar 12. PubMed PMID: 21470695.
  • Saha D, Lefebvre DJ, Van Doorsselaere J, Atanasova K, Barbé F, Geldhof M, Karniychuk UU, Nauwynck HJ. Pathologic and virologic findings in mid-gestational porcine foetuses after experimental inoculation with PCV2a or PCV2b. Vet Microbiol. 2010 Sep 28;145(1-2):62-8. doi: 10.1016/j.vetmic.2010.03.017. Epub 2010 Mar 27. PubMed PMID: 20395077.
  • Karniychuk UU, Geldhof M, Vanhee M, Van Doorsselaere J, Saveleva TA, Nauwynck HJ. Pathogenesis and antigenic characterization of a new East European subtype 3 porcine reproductive and respiratory syndrome virus isolate. BMC Vet Res. 2010 Jun 4;6:30. doi: 10.1186/1746-6148-6-30. PubMed PMID: 20525333; PubMed Central PMCID: PMC2898778.
  • Karniychuk UU, Nauwynck HJ. Quantitative changes of sialoadhesin and CD163 positive macrophages in the implantation sites and organs of porcine embryos/fetuses during gestation. Placenta. 2009 Jun;30(6):497-500. doi: 10.1016/j.placenta.2009.03.016. Epub 2009 May 2. PubMed PMID: 19410291.