People

Gireesh Rajashekara

DVM, PhD

Professor 

Contact

rajashekara.2@osu.edu (330) 263-3745 Food Animal Health Building
1680 Madison Ave.
Wooster, OH 44691
Map Link

Department

Veterinary Preventive Medicine

Related Links

Professional Training and Experience

  • BVSc(DVM), Veterinary Medicine, University of Agricultural Sciences, Karnataka, India
  • MVSc, Microbiology, University of Agricultural Sciences, Karnataka, India
  • PhD, Microbiology, University of Minnesota, Minnesota, USA
  • Post doctoral Training, Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA and Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI

Research Interests

  • Molecular microbiology
  • Host-pathogen interactions and pathogenesis
  • Virulence mechanisms
  • Vaccine Development

University Awards and Honors

Patents

  • Application of recombinant fimbrial protein antigen for the diagnosis of Salmonella enteritidis infection in poultry United States Patent 6,495,334 and International Patent WO 98/03656
  • Brucella melitensis mutants and methods Patent PO6114US (pending)

Professional Memberships

  • American Society for Microbiology
  • American Association of Immunologists

Publications

2020

  • Garmendia, A.E, W. Mwangi and G.J. Renukaradhya. Chapter 29 – Porcine Reproductive and Respiratory Syndrome. Veterinary Vaccines for Livestock - 1st Edition, FAO9 Editors - Samia Metwally, Ahmed Elldrissi and Gerrit Viljoen, Elsevier Publication 2020.
  • Renu, S, A.D. Markazi, S. Dhakal, Y.S. Lakshmanappa, R. Shanmugasundaram, R.K. Selvaraj and G.J. Renukaradhya* (2020). Oral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens. Int J Nanomedicine, 15:761-777. PMID: 32099364.
  • Hogshead, B, J. Pempek, S. Dhakal, S. Ghimire, R. Sankar, K. Proudfoot, G. Habing and G.J. Renukaradhya* (2020). Bovine parainfluenza-3 antibodies in veal calves supplemented with cinnamaldehyde or lactoferrin. Applied Animal Science, 36(1):118-123.
  • Renu, S, N. Feliciano-Ruiz, S. Ghimire, Y. Han, J. Schrock, S. Dhakal, V. Patil, S. Krakowka and G.J. Renukaradhya* (2020). Poly(I:C) augments inactivated influenza virus-chitosan nanovaccine induced cellular immune response in intranasal vaccinated pigs. Vet Microbiology, 242:108611. PMID: 32122615.
  • Hiremath, J, K. Tabynov and G.J. Renukaradhya* (2020). Pulmonary Inflammatory Response to Influenza Virus Infection is regulated by DAP12 and macrophage M1 and M2 phenotypes: a study in a porcine model. Cellular Immunology, 352:104078. PMID: 32164997.
  • Mailybayeva, A, S. Ryskeldinova, N. Zinina, E-M, Zhou, G.J. Renukaradhya and K. Tabynov (2020). Evaluation of duration of immunogenicity and protective efficacy of improved influenza viral vector-based B. abortus vaccine against B. melitensis infection in sheep and goats. Front Vet Science, 7:58. PMID: 32175335.
  • Akerele, G, N. Ramadan, S. Renu, G.J. Renukaradhya, R. Shanmugasundaram, R.K. Selvaraj (2020). In vitro characterization and immunogenicity of chitosan nanoparticles loaded with native and inactivated extracellular proteins from a field strain of Clostridium perfringens associated with necrotic enteritis. Vet Immunol Immunopathol., 224:110059. PMID: 32408182.
  • Han, Y, S. Renu, V. Patil, J. Schrock, N. Feliciano-Ruiz, R. Selvaraj, and G.J. Renukaradhya* (2020). Mannose-Modified Chitosan-Nanoparticle-Based Salmonella Subunit Oral Vaccine-Induced Immune Response and Efficacy in a Challenge Trial in Broilers. Vaccines (Basel), 8(2):E299. PMID: 32545295.
  • Han, Y, S. Renu, V. Patil, J. Schrock, N. Feliciano-Ruiz, R. Selvaraj, and G.J. Renukaradhya* (2020). Immune Response to Salmonella Enteritidis Infection in Broilers Immunized Orally With Chitosan-Based Salmonella Subunit Nanoparticle Vaccine. Front Immunol., 11:935. PMID: 32508828.
  • Renu, S, Y. Han, S. Dhakal, Y.S. Lakshmanappa, S. Ghimire, N. Feliciano-Ruiz, S. Senapati, B. Narasimhan, R.K. Selvaraj and G.J. Renukaradhya* (2020). Chitosan-adjuvanted Salmonella subunit nanovaccine for poultry delivered through drinking water and feed. Carbohydrate Polymer, 243:116434. PMID: 32532387.
  • Renu, S, N. Feliciano-Ruiz, F. Lu, S. Ghimire, Y. Han, J. Schrock, S. Dhakal, V. Patil, S. Krakowka, H. HogenEsch and G.J. Renukaradhya* (2020). A Nanoparticle-Poly(I:C) Combination Adjuvant Enhances the Breadth of the Immune Response to Inactivated Influenza Virus Vaccine in Pigs. Vaccines (Basel), 8(2):E229. PMID: 32443416.
  • Han, Y, Renu, S, J. Schrock, K.Y. Acevedo-Villanuev, B. Lester, R.K. Selvaraj, and G.J. Renukaradhya* (2020). Temporal dynamics of innate and adaptive immune responses in broiler birds to oral delivered chitosan-based Salmonella subunit nanoparticle vaccine. Vet Immunol and Immunopathology, 228:110111). PMID: 32846353.
  • Patil, V, S. Renu, N. Feliciano-Ruiz, Y. Han, A. Ramesh, J. Schrock, S. Dhakal, H. HogenEsch and G.J. Renukaradhya* (2020). Intranasal delivery of inactivated influenza virus and Poly(I:C) adsorbed corn-based nanoparticle vaccine elicited robust antigen-specific cell- mediated immune responses in maternal antibody positive nursery pigs. Front Immunol., 11:596964. PMID:33391267.
  • Srivastava V, Deblais L, Huang HC, Miyazaki A, Kandasamy S, Langel SN, Paim FC, Chepngeno J, Kathayat D, Vlasova AN, Saif LJ, Rajashekara G. Reduced rotavirus vaccine efficacy in protein malnourished human-faecal-microbiota-transplanted gnotobiotic pig model is in part attributed to the gut microbiota. Beneficial Microbes. 2020 Dec 2;11(8):733-751. doi: 10.3920/BM2019.0139.
  • Rajashekara G, Deblais L, Miller SA. Impact of plant pathogens infection on  Salmonella  enterica subsp. enterica serotype Typhimurium persistence in tomato plants. J Food Prot. 2020 Nov 12. doi: 10.4315/JFP-20-291.
  • Kathayat D, Antony L, Deblais L, Helmy YA, Scaria J, Rajashekara G. 2020. Small molecule adjuvants potentiate colistin activity and attenuate resistance development against Escherichia coli by affecting pmrAB system. Infection & Drug Resistance 2020;13:2205-2222
  • Helmy YA, Kathayat D, Ghanem M, Jung K, Closs Jr G, Deblais D, Srivastava V, El-Gazzar M, and Rajashekara G. 2020. Identification and characterization of novel small molecule inhibitors to control Mycoplasma gallisepticum infection in chickens. Veterinary Microbiology 247,108799. DOI:10.1016/j.vetmic.2020.108799.
  • Lu Y, Deblais L, Rajashekara G, Miller SA, Helmy YA, Zhang H, Wu P, Qiu Y, Xu X. 2020. High-throughput screening reveals small molecule modulators inhibitory to Acidovorax citrulli. Plant Pathol. 69 (5) 818-826. https://doi.org/10.1111/ppa.13177
  • Michael H, Langel SN, Miyazaki A, Paim FC, Chepngeno J, Alhamo MA, Fischer DD, Srivastava V, Kathayat D, Deblais L, Rajashekara G, Saif LJ, Vlasova AN. 2020. Malnutrition Decreases Antibody Secreting Cell Numbers Induced by an Oral Attenuated Human Rotavirus Vaccine in a Human Infant Fecal Microbiota Transplanted Gnotobiotic Pig Model. Front Immunol 11.
  • Terefe Y, Deblais L, Ghanem M, Helmy YA, Mummed B, Chen D, Singh N, Ahyong V, Kalantar K, Yimer G, Yousuf Hassen J, Mohammed A, McKune SL, Manary MJ, Ordiz MI, Gebreyes W, Havelaar AH, Rajashekara G. 2020. Co-occurrence of Campylobacter Species in Children From Eastern Ethiopia, and Their Association With Environmental Enteric Dysfunction, Diarrhea, and Host Microbiome. Front Public Health 8. Front Public Health. 2020 Apr 15;8:99. doi: 10.3389/fpubh.2020.00099.
  • Vrisman CM, Deblais L, Helmy YA, Johnson R, Rajashekara G, Miller SA. 2020. Discovery and Characterization of Low-Molecular Weight Inhibitors of Erwinia tracheiphila. Phytopathology® 110:989–998.
  • Chen D, McKune SL, Singh N, Hassen JY, Gebreyes W, Manary M, Bardosh K, Yang Y, Diaz N, Mohammed A, Terefe Y, Roba KT, Ketema M, Ameha N, Assefa N, Rajashekara G, Deblais L, Ghanem M, Yimer G, Ordiz I, Havelaar AH. 2021. Campylobacter Colonization, Environmental Enteric Dysfunction, Stunting, and Associated Risk Factors among Young Children in Rural Ethiopia: a Cross-sectional Study from the Campylobacter Genomics and Environmental Enteric Dysfunction (CAGED) Project. Front. Public Health, 21 January 2021. doi.org/10.3389/fpubh.2020.615793.
  • Helmy Y.A., Kassem I.I., and Rajashekara G. (2021). Immuno-modulatory effect of probiotic E. coli Nissle 1917 in polarized human colonic cells against Campylobacter jejuni infection. Gut Microbes 13 (1), 1-16.
    DOI.org/10.1080/19490976.2020.1857514.

2019

  • Garmendia, A.E, W. Mwangi and G.J. Renukaradhya. Chapter 29 – Porcine Reproductive and Respiratory Syndrome. Veterinary Vaccines for Livestock - 1st Edition, FAO. Editors - Samia Metwally, Ahmed Elldrissi and Gerrit Viljoen, Elsevier Publication 2019.
  • Dhakal, S, F. Lu, S. Ghimire, S. Renu, Y.S. Lakshmanappa, B. Hogshead, D. Ragland, H., HogenEsch and G.J. Renukaradhya (2019). Corn-derived alpha-D-glucan nanoparticles as adjuvant for intramuscular and intranasal immunization in pigs. Nanomedicine: Nanotechnology, Biology, and Medicine, 16:226-235. PMID: 30611772, IF – 5.005.
  • Nurpeisova, A, M. Kassenov, N. Rametov, K. Tabynov, G. J. Renukaradhya, Y. Volgin, A. Sagymbay, G. Sarsenbayeva, N. Bogdanov, T. Isagilov, A. Makbuz, A. Sansyzbay and B. Khairullin (2019). Analysis of Efficacy of Adjuvant-based Inactivated Pandemic H5N1 Influenza Virus Vaccine. Archives of Virology, 164(4):1027-1036, IF – 2.261.
  • Dhakal, S, L. Wang, L. Antony, J. Rank, P. Bernardo, S. Ghimire, K. Bondra, C. Siems, Y.S. Lakshmanappa, S. Renu, B. Hogshead, S. Krakowka, M. Kauffman, J. Scaria, J.T. LeJeune, Z. Yu and G.J. Renukaradhya (2019). Amish (Rural) vs. non-Amish (Urban) Infant Fecal 6 Microbiotas Are Highly Diverse and Their Transplantation Lead to Differences in Mucosal Immune Maturation in a Humanized Germfree Piglet Model. Frontiers in Immunology, 10:1509. PMID: 31379808, IF – 5.511.
  • Dhakal, S, K.A. Ross, S. Ghimire, Y.S. Lakshmanappa, B. Hogshead, P. Bernardo, C.W. Lee, M.J. Wannemuehler, B. Narasimhan and G.J. Renukaradhya (2019). Evaluation of CpG-ODN-adjuvanted polyanhydride-based intranasal influenza nanovaccine in pigs. Veterinary Microbiology, 226:23-30. PMID: 31585639, IF – 2.524.
  • Renu, S, A.D. Markazi, S. Dhakal, Y.S. Lakshmanappa, R. Shanmugasundaram, R.K. Selvaraj and G.J. Renukaradhya (2019). Formulation of oral mucoadhesive chitosan-Salmonella subunit nanovaccine for layer birds. International Journal of Nanomedicine (In Press). IF – 4.370.
  • Hogshead, B, J. Pempek, S. Dhakal, S. Ghimire, R. Sankar, K. Proudfoot, G. Habing and G.J. Renukaradhya (2019). Bovine parainfluenza-3 antibodies in veal calves supplemented with cinnamaldehyde or lactoferrin. Applied Animal Science (In Press).
  • Deblais L, Helmy YA, Kathayat D, Huang HC, Miller SA, Rajashekara G. Novel Imidazole and Methoxybenzylamine Growth Inhibitors Affecting Salmonella Cell Envelope Integrity and its Persistence in Chickens. Sci Rep. 2019 Nov 6;9(1):16508. doi: 10.1038/s41598-019-52826-x. PubMed PMID: 31695121; PubMed Central PMCID: PMC6834860.
  • Vlasova AN, Takanashi S, Miyazaki A, Rajashekara G, Saif LJ. How the gut microbiome regulates host immune responses to viral vaccines. Curr Opin Virol. 2019 Aug; 37:16-25. doi: 10.1016/j.coviro.2019.05.001. Epub 2019 Jun 1. Review. PubMed PMID: 31163292.
  • Deblais L, Vrisman C, Kathayat D, Helmy YA, Miller SA, Rajashekara G. Imidazole and Methoxybenzylamine Growth Inhibitors Reduce Salmonella Persistence in Tomato Plant Tissues. J Food Prot. 2019 Jun;82(6):997-1006. doi:10.4315/0362-028X. JFP-18-555. PubMed PMID: 31121102.
  • Deblais L, Helmy YA, Testen A, Vrisman C, Jimenez Madrid AM, Kathayat D, Miller SA, Rajashekara G. Specific Environmental Temperature and Relative Humidity Conditions and Grafting Affect the Persistence and Dissemination of Salmonella enterica subsp. enterica Serotype Typhimurium in Tomato Plant Tissues. Appl Environ Microbiol. 2019 May 16;85(11). pii: e00403-19. doi:10.1128/AEM.00403-19. Print 2019 Jun 1. PubMed PMID: 30926732; PubMed Central, PMCID: PMC6532026.
  • Deblais L, Helmy YA, Kumar A, Antwi J, Kathayat D, Acuna UM, Huang HC, de
  • Blanco EC, Fuchs JR, Rajashekara G. Novel narrow spectrum benzyl thiophene sulfonamide derivatives to control Campylobacter. J Antibiot (Tokyo). 2019 Jul;72(7):555-565. doi: 10.1038/s41429-019-0168-x. Epub 2019 Mar 27. PubMed PMID:30918323.

2018

  • Garmendia, A.E, W. Mwangi and G.J. Renukaradhya. Chapter 29 – Porcine Reproductive and Respiratory Syndrome. Veterinary Vaccines for Livestock - 1st Edition, FAO. Editors - Samia Metwally, Ahmed Elldrissi and Gerrit Viljoen, Elsevier Publication 2018. 
  • Liu, J, R.M. Gallo, M. Khan, G.J. Renukaradhya and R.R. Brutkiewicz (2018). Neurofibromin 1 Impairs Natural Killer T-Cell-Dependent Antitumor Immunity against a T Cell Lymphoma. Front. Immunol., 8:1901. PMID: 29354122, IF – 5.511. 
  • Li, Y, P. Shang, C. Carrillo, P. Naraghi-Arani, C.J. Jain, G.J. Renukaradhya, A.W. Firth, E.J. Snijder and Y. Fang* (2018). Nonstructural proteins nsp2TF and nsp2N of porcine reproductive and respiratory syndrome virus (PRRSV) play important roles in suppressing host innate immune responses. Virology, 517:164-176. PMID: 29325778, IF – 3.374. 
  • Renu, S, S. Dhakal, E. Kim, J. Goodman, Y.S. Lakshmanappa, M.J. Wannemuehler, B. Narasimhan, P.B. Boyaka, and G.J. Renukaradhya (2018). Intranasal delivery of influenza antigen by nanoparticles, but not NKT-cell adjuvant differentially induces the expression of B-cell activation factors in mice and swine. Cellular Immunology, 329:27-30. PMID: 29665972, IF – 2.995. 
  • Dhakal, S, S. Renu, S. Ghimire, Y.S. Lakshmanappa, B. Hogshead, N.F. Ruiz, F. Lu, H. HogenEsch, S. Krakowka, C.W. Lee and G.J. Renukaradhya (2018). Mucosal immunity and protective efficacy of intranasal inactivated influenza vaccine is improved by chitosan nanoparticle delivery in pigs. Frontiers in Immunology, 9:934. PMID: 29770135, IF – 5.511.
  • Dhakal, S, X. Cheng, J. Salcido, S. Renu, K. Bondra, Y.S. Lakshmanappa, C. Misch, S. Ghimire, N.F. Ruiz, B. Hogshead, S. Krakowka, K. Carson, J. McDonough, C.W. Lee and G.J. Renukaradhya (2018). Liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs. International Journal of Nanomedicine, 13:6699-6715. PMID: 30425484, IF – 4.370. 
  • Zhugunissov, K, Y. Bulatov, D. Taranov, Z. Yershebulov, Z. Koshemetov, A. Zhunushov, G.J. Renukaradhya, K. Tabynov and Y. Abduraimov (2018). Beta-propiolactone inactivated bivalent bluetongue virus vaccine containing Montanide ISA-71VG adjuvant induces long-term immune response in sheep against serotypes 4 and 16 even after 3 years of controlled vaccine storage. Veterinary Microbiology, 226:23-30. PMID: 30389040, IF – 2.524. 
  • Guo R, P. Shang P, C.A. Carrillo, Z. Sun, Y.S. Lakshmanappa, X. Yan, G.J. Renukaradhya, J. McGill, C.J. Jaing, M.C. Niederwerder, R.R.R. Rowland and Y. Fang (2018). Double-stranded viral RNA persists in vitro and in vivo during prolonged infection of porcine reproductive and respiratory syndrome virus. Virology, 524:78-89. PMID: 30165309, IF – 3.374. 
  • Renu, S, A.D. Markazi, S. Dhakal, Y.S. Lakshmanappa, S.R. Gourapura, R. Shanmugasundaram, S. Senapati, B. Narasimhan, R.K. Selvaraj and G.J. Renukaradhya (2018). Surface engineered polyanhydride based oral Salmonella subunit nanovaccine for poultry. International Journal of Nanomedicine, 13:8195-8215. PMID: 30555234, IF – 4.370. Dhakal, S, F. Lu, S. Ghimire, S. Renu, Y.S. Lakshmanappa, B. Hogshead, D. Ragland, H., HogenEsch and G.J. Renukaradhya (2018). Corn-derived alpha-D-glucan nanoparticles as adjuvant for intramuscular and intranasal immunization in pigs. Nanomedicine, pii: S1549-9634(18)30570-7. doi: 10.1016/j.nano.2018.12.003. PMID: 30611772, IF – 5.005
  • Miyazaki, A., Kandasamy, S., Michael, H., Langel, S.N., Paim, F.C., Chepngeno, J., Alhamo, M.A., Fischer, D.D., Huang, H.C., Srivastava, V., Kathayat, D., Deblais, L., Rajashekara, G., Saif, L.J., Vlasova, A.N. 2018. Protein deficiency reduces efficacy of oral attenuated human rotavirus vaccine in a human infant fecal microbiota transplanted gnotobiotic pig model. Submitted to Vaccine. Vaccine. 2018 Oct 8;36(42):6270-6281.
  • Kumar, A., Vlasova, A., Deblais, L., Huang, H.C., Wijeratne, A., Kandasamy, S., Fischer, D.D., Langel, S.N., Paim, F.C., Alhamo, M.A., Shao, L., Saif, L.J., Rajashekara, G. 2018. Impact of nutrition and rotavirus infection on the infant gut microbiota in a humanized pig model. BMC Gastroenterol. 2018 Jun 22;18(1):93. doi: 10.1186/s12876-018-0810-2. PMC N/A
  • Huang, H.C., Vlasova, A.N., Kumar, A., Kandasamy, S., Fischer, D.D., Deblais, L., Paim, F.C., Langel, S.N., Alhamo, M.A., Rauf, A., Shao, L., Saif, L.J., Rajashekara, G. 2018. Effect of antibiotic, probiotic, and human rotavirus infection on colonisation dynamics of defined commensal microbiota in a gnotobiotic pig model. Benef Microbes. 2017 Oct 12:1-16. doi: 10.3920/BM2016.0225.

2017

  • Garmendia, A.E, W. Mwangiand Gourapura, R.J. Chapter 29 –Porcine Reproductive and Respiratory Syndrome. Veterinary Vaccines for Livestock -1st Edition, FAO. Editors -Samia Metwally, Ahmed Elldrissi and Gerrit Viljoen, Elsevier Publication 2018.
  • Mailybayeva, A.; Yespembetov, B.; Ryskeldinova, S.; Zinina, N.; Gourapura, R.J.; Petrovsky, N.; Tabynov, K. (2017). Improved influenza viral vector based Brucella abortus vaccine induces robust B and T-cell responses and protection against Brucella melitensis infection in pregnant sheep and goats. PLOS ONE, (10), e0186484-e0186484. doi:10.1371/journal.pone.0186484 Publication status: Published, Percent of authorship: 10%, Description of effort:  Analyzed the results and edited the manuscript. Impact factor -2.806.
  • Dhakal, S.; Goodman, J.; Bondra, K.; Lakshmanappa, Y.S.; Gourapura, R.J. (2017). Polyanhydride nanovaccine against swine influenza virus in pigs. Vaccine, (8), 1124-1131. doi:10.1016/j.vaccine.2017.01.019
  • Publication status: Published, Percent of authorship: 50%, Description of effort:  Designed the research, performed the experiments, analyzed the results and edited the manuscript. Impact factor -3.235.
  • Elaish, M.; Ngunjiri, J.M.; Ali, A.; Xia, M.; Gourapura, R.J. (2017). Supplementation of inactivated influenza vaccine with norovirus P particle-M2e chimeric vaccine enhances protection against heterologous virus challenge in chickens. PloS one, (2), e0171174. doi:10.1371/journal.pone.0171174. Publication status: Published, Percent of authorship: 10%, Description of effort: Analyzed the results and edited the manuscript. Impact factor -2.806.
  • Dhakal, S, J. Hiremath, K. Bondra, Y.S. Lakshmanappa, D. Shyu, K. Oyuang, K. Kang, B. Binjawadagi, J. Goodman, K. Tabynov, S. Krakowka, B. Narasimhan, C.W. Lee and Gourapura, R.J. (2017). Biodegradable nanoparticle delivery of inactivated swine influenza virus vaccine provides heterologous cell-mediated immune response in pigs. Journal of Controlled Release, 247, 194-205. Publication status: Published, Percent of authorship: 50%, Description of effort: Designed the experiments, performed the study, analyzed the results and edited the manuscript. Impact factor -7.441.
  • Renur, OMarkazi, A.D.; Selvaraj, R.; Gourapura, R.J. (2017). Engineering of Targeted Mucoadhesive Chitosan Based Salmonella Nanovaccine for Oral Delivery in Poultry. Manuscript Submitted.
  • Dhakal, S.; Cheng, X.; Salcido, J.; Lee, C.W.; Gourapura, R.J.  (2017). Liposome nanoparticle-based influenza conserved peptides vaccine and monosodium urate crystal adjuvant elicits protective immune response in pigs. Manuscript under Revision.
  • Isaac P. Kashoma, Vishal Srivastava and Gireesh Rajashekara, Advances in Vaccines for controlling Campylobacter in Poultry. In, Food Safety in Poultry Meat Production. Editors: Dr. Siddhartha Thakur and Dr. Steven Ricke and Kumar Venkitanarayanan. Springer Nature. Under review
  • Issmat I. Kassem and Gireesh Rajashekara. Campylobacter in Poultry. In Diagnosis of major poultry diseases. 2017. Editors: Libro de Toro, Haroldo; Saif, Yehia Mo. Servet Publishing. ISBN: 8417225056 ISBN-13: 9788417225056
  • Heshan Du, Bin Chen, Xiaofen Zhang, and Fenglan Zhang, Sally A. Miller, Gireesh Rajashekara, Xiulan Xu and Sansheng Geng, Evaluation of Ralstonia solanacearum Infection Dynamics in Resistant and Susceptible Pepper Lines Using Bioluminescence Imaging. Plant Disease /Vol. 101 No. 2; 272-278. February 2017
  • Huang HC, Vlasova AN, Kumar A, Kandasamy S, Fischer DD, Deblais L, Paim FC, Langel SN, Alhamo MA, Rauf A, Shao L, Saif LJ, Rajashekara G. Effect of antibiotic, probiotic, and human rotavirus infection on colonisation dynamics of defined commensal microbiota in a gnotobiotic pig model. Benef Microbes. 2018 Jan 29;9(1):71-86. doi: 10.3920/BM2016.0225. Epub 2017 Oct 12.PMID: 29022385
  • Helmy YA, Kassem II, Kumar A, Rajashekara G. In Vitro Evaluation of the Impact of the Probiotic E. coli Nissle 1917 on Campylobacter jejuni's Invasion and Intracellular Survival in Human Colonic Cells.Front Microbiol. 2017 Aug 22;8:1588. doi: 10.3389/fmicb.2017.01588. eCollection 2017. PMID: 28878749
  • Amadi VA, Matthew-Belmar V, Subbarao C, Kashoma I, Rajashekara G, Sharma R, Hariharan H, Stone D. Campylobacter Species Isolated from Pigs in Grenada Exhibited Novel Clones: Genotypes and Antimicrobial Resistance Profiles of Sequence Types.Foodborne Pathog Dis. 2017 Jul;14(7):419-425. doi: 10.1089/fpd.2016.2229. Epub 2017 Jun 26. PMID: 28650672
  • Fischer DD, Kandasamy S, Paim FC, Langel SN, Alhamo MA, Shao L, Chepngeno J, Miyazaki A, Huang HC, Kumar A, Rajashekara G, Saif LJ, Vlasova AN. Protein Malnutrition Alters Tryptophan and Angiotensin-Converting Enzyme 2 Homeostasis and Adaptive Immune Responses in Human Rotavirus-Infected Gnotobiotic Pigs with Human Infant Fecal Microbiota Transplant. Clin Vaccine Immunol. 2017 Aug 4;24(8). pii: e00172-17. doi: 10.1128/CVI.00172-17. Print 2017 Aug. PMID: 28637803
  • Kassem II, Helmy YA, Kathayat D, Candelero-Rueda RA, Kumar A, Deblais L, Huang HC, Sahin O, Zhang Q, Rajashekara G. Nonculturability Might Underestimate the Occurrence of Campylobacter in Broiler Litter. Foodborne Pathog Dis. 2017 Aug;14(8):472-477. doi: 10.1089/fpd.2017.2279. Epub 2017 Jun 16. PMID: 28622473
  • Nisar M, Kassem II, Rajashekara G, Goyal SM, Lauer D, Voss S, Nagaraja KV. Genotypic relatedness and antimicrobial resistance of Salmonella Heidelberg isolated from chickens and turkeys in the midwestern United States. J Vet Diagn Invest. 2017 May;29(3):370-375. doi: 10.1177/1040638717690784. PMID: 28430086
  • Vlasova AN, Paim FC, Kandasamy S, Alhamo MA, Fischer DD, Langel SN, Deblais L, Kumar A, Chepngeno J, Shao L, Huang HC, Candelero-Rueda RA, Rajashekara G, Saif LJ. Protein Malnutrition Modifies Innate Immunity and Gene Expression by Intestinal Epithelial Cells and Human Rotavirus Infection in Neonatal Gnotobiotic Pigs. mSphere. 2017 Mar 1;2(2). pii: e00046-17. doi: 10.1128/mSphere.00046-17. eCollection 2017 Mar-Apr. PMID: 28261667
  • Kassem II, Candelero-Rueda RA, Esseili KA, Rajashekara G. Formate simultaneously reduces oxidase activity and enhances respiration in Campylobacter jejuni. Sci Rep. 2017 Jan 16;7:40117. doi: 10.1038/srep40117. PMID: 28091524
  • Vrisman, C.M., Deblais, L., Rajashekara, G., Miller, S.A. Discovery and characterization of small molecules inhibitory to Erwinia tracheiphila. Phytopathology 107:S5.42
  • Kandasamy S, Vlasova AN, Fischer DD, Chattha KS, Shao L, Kumar A, Langel SN, Rauf A, Huang HC, Rajashekara G, Saif LJ. Unraveling the Differences between Gram-Positive and Gram-Negative Probiotics in Modulating Protective Immunity to Enteric Infections. Front Immunol. 2017 Mar 27;8:334. doi: 10.3389/fimmu.2017.00334. eCollection 2017. Review. PMID: 28396664
  • Chandrashekhar K, Kassem II, Rajashekara G. Campylobacter jejuni transducer like proteins: Chemotaxis and beyond. Gut Microbes. 2017 Jul 4;8(4):323-334. doi: 10.1080/19490976.2017.1279380. Epub 2017 Jan 12. PMID:28080213.
  • Zilu Wan, Gireesh Rajashekara, James Fuchs, Esperanza Carcache de Blanco, Melvin A. Pascall. Incorporation of Selected Small Molecule Compounds into Tapioca Starch and the Effects on Thickness, Moisture and Oxygen Mass Transfer, and Mechanical Properties of the Films. Packaging Technology and Science (In Press).
  • Loïc Deblais, Yosra A. Helmy, Diapak Kathayat, Rosario A Candelero, Huang-chi Huang, Sally A. Miller, Gireesh Rajashekara. Novel Small Molecules Growth Inhibitor Affecting Salmonella Cell Membrane and Effective in Chicken Broilers. Submitted to AAC.
  • Loïc Deblais, Ben Lorentz, Joy Scaria, Kakambi V. Nagaraja, Muhammad Nisar, and Gireesh Rajashekara. Divergent Metabolic Functions in Salmonella Heidelberg Isolated from Chicken and Turkey Production Systems. Submitted to AEM.
  • Anand Kumar; Ananstasia Vlasova; Loic Deblais; Huang-Chi Huang; Asela Wijeratne; Sukumar Kandasamy; David D Fischer; Stephanie N Langel; Francine Chimelo Paim; Moyasar A. Alhamo A Alhamo; Lulu Shao; Linda J Saif; Gireesh Rajashekara. Impact of nutrition and rotavirus infection on the infant gut microbiota in a humanized pig model. BMC Gastroenterology

2016

  • Tabynov, K, B. Yespembetov, N. Matikhan, S. Ryskeldinova, N. Zinina, Z. Kydyrbayev, N. Assanzhanova, K. Tabynov, G.J. Renukaradhya and A. Sansyzbay, "First evaluation of an influenza viral vector based Brucella abortus vaccine in sheep and goats: assessment of safety, immunogenicity and protective efficacy against Brucella melitensis infection.". Vet. Microbiology. Vol. 197, 15-20. 2016. (Published) 25% Authorship: 25% Authorship. Analyzed the results and edited the manuscript. Impact factor - 2.511
  • Tabynov, K, M. Orynbayev, G.J. Renukaradhya and A. Sansyzbay, "Simultaneous subcutaneous and conjunctival administration of the influenza viral vector Brucella abortus vaccine to pregnant heifers provides better protection against B. abortus 544 infection than the commercial B. abortus S19 vaccine.". Vaccine. Vol. 34, no. 42: 5049-52. 2016. (Published) 25% Authorship: 25% Authorship. Analyzed the results and edited the manuscript. Impact factor - 3.413
  • Tabynov, K, Sansyzbay A, Tulemissova, Z, Dhakal, S, Renukaradhya, GJ, and Mambetaliyev, M, "Inactivated porcine reproductive and respiratory syndrome virus vaccine adjuvanted with MontanideTM Gel 01 ST elicits virus-specific cross-protective inter-genotypic response in piglets". Veterinary Microbiology. Vol. 192, 81-89. 2016. (Published) 50% Authorship: Analyzed the results and edited the manuscript. Impact factor - 2.511.
  • Dwivedi V, C. Manickam, S. Dhakal, B. Binjawadagi, K. Oyuang, J. Hiremath, M. Khatri, J. Gervey-Hague, C-W. Lee, and G.J. Renukaradhya, "Adjuvant effects of invariant NKT cell ligand potentiates the innate and adaptive immunity to an inactivated H1N1 swine influenza virus vaccine in pigs". Veterinary Microbiology. Vol. 186, 157-163. 2016. (Published) 50% Authorship: Designed the experiments, performed the study, analyzed the results and edited the manuscript. Impact factor - 2.511.
  • Hiremath J, Kyung-il K, Elaish M, Binjawadagi B, Ouyang K, Dhakal S, Torrelles JB, Lee C-W, and Renukaradhya GJ, "Entrapment of H1N1 influenza virus derived conserved peptides in PLGA nanoparticles enhances T cell response and vaccine efficacy in pigs". PLOS One. Vol. 11, no. 4: e0151922. 2016. (Published) 50% Authorship: Designed the experiments, performed the study, analyzed the results and edited the manuscript. Impact factor - 3.234.
  • Ouyang, K, J. Hiremath, B. Binjawadagi, D-L. Shyu, S. Dhakal, J. Arcos, R. Schleappi, L. Holman, M. Roof, J.B. Torrelles, and G.J. Renukaradhya, "Comparative analysis of routes of immunization of a live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in a heterologous virus challenge study". Veterinary Research. Vol. 47, no. 1: 45. 2016. (Published) 50% Authorship: Designed the experiments, performed the study, analyzed the results and edited the manuscript. Impact factor - 2.82.
  • Yanhua, L, Shyu, D-L, Shang, P, Bai, J, Ouyang, K, Dhakal, S, Hiremath, JH, Binjawadagi, B, Renukaradhya, GJ, and Fang, Y., "Mutations in a highly conserved motif of nsp1β protein attenuate the innate immune suppression function of porcine reproductive and respiratory syndrome virus (PRRSV)". Journal of Virology. Vol. 90, no. 7: 3584-3599. 2016. (Published) 50% Authorship: Corresponding authors: Renukaradhya, G.J., and Fang, Y. Designed the experiments, performed part of the study, analyzed the results and edited the manuscript. Impact factor - 5.076.

2015

  • Thomas, M, Wang, Z, Sreenivasan, CC, Hause, BM, Renukaradhya, GJ, Li, F, Francis, DH, Kaushik, RS, Khatri, M., "Poly I:C adjuvanted inactivated swine influenza vaccine induces heterologous protective immunity in pigs". Vaccine. Vol. 33, no. 4: 542-548. 2015.
  • Ouyang, K, Shyu, D-L, Dhakal, S, Hiremath, J, Binjawadagi, B, Yashavanth, SL, Guo, R, Ransburgh, R, Bondra, KM, Gauger, P, Zhang, J, Specht, T, Gilbertie, A, Minton, W, Fang, Y, and Renukaradhya, GJ., "Evaluation of humoral immune status in porcine epidemic diarrhea virus (PEDV) infected sows under field conditions". Veterinary Research. Vol. 46, no. 1: 140. 2015.
  • Renukaradhya, GJ, Meng, X-J, Calvert, JG, Roof, M, and Lager, KM., "Inactivated and subunit vaccines against porcine reproductive and respiratory syndrome: Current status and future direction". Review of Inactivated and subunit vaccines against porcine reproductive and respiratory syndrome, by Veterinary vaccine researchers. Vaccine. Vol. 33, no. 27: 3065-3072. (2015).
  • Renukaradhya, GJ, Meng, X-J, Calvert, JG, Roof, M, and Lager, KM., "Live porcine reproductive and respiratory syndrome virus-based vaccines: current status and future direction". Review of Live porcine reproductive and respiratory syndrome virus candidate vaccines, by Veterinary vaccine researchers. Vaccine. Vol. 33, no. 33: 4069- 4080. (2015).
  • Lunney, JK, Fang, Y, Ladinig, A, Chen, N, Li, Y, Rowland, B, and Renukaradhya, GJ., "Porcine reproductive and respiratory syndrome virus (PRRSV): Pathogenesis and Interaction with the Immune System". Review of PRRSV Pathogenesis and Interaction with the Immune System, by Particulate vaccine delivery researchers. Annual Review of Animal Biosciences. Vol. 14, 25. (2015).
  • Renukaradhya, GJ, Narasimhan, B, and Mallapragada, SK, "Respiratory nanoparticle-based vaccines and challenges associated with animal models and translation". Review of Nanoparticle based vaccine delivery system in animal models, by Particulate vaccine delivery researchers. Journal of Control Release. Vol. 219, 622-631. (2015)
  • Sukumar Kandasamy. Anand Kumar, Gireesh Rajashekara, Anastasia Vlasova, Linda Saif., "Probiotics for Immunomodulation and treatment of Immunological disorders in children: Evaluation in a germ free animal model.". In "Probiotics in Children". Edited by: Marco Manfredi and Gian Luigi de'Angelis. New York: Nova Science Publishers.2015.
  • Kashoma IP, Kassem Il, Julius John J, Kessy BMs Gebreyes W, Kazwala RR, and Rajashekara G. "Prevalence and Antibiotic Resistance of Campylobacter Isolated from Dressed Cattle Carcasses and Raw Milk in Tanzania". Microbial Drug Resistance. Vol. 22, no. 1: 40-52. 2015.
  • Chandrashekhar K, Gangaiah DI Mimbela R, Kassem Il, Jeon B and Rajashekara G., 'Transducer like proteins of Campylobacter jejuni 81-176: rote in chemotaxis and colonization of the chicken gastrointestinal tract". Front. Cell. Infect. Microbiol. Vol. May 201:1-10. 2015.
  • Xu X, Kumar A. Deblais L, Pina-Mímbela R, Nistow C. Fuchs JR Miller SAI Rajashekara G., "Discovery of novel small molecule modulators of Ctavibacter michiganensis subsp. michiganensis,". Front Microbiol. Vol. 6, el 127. 2015.
  • Kashoma IP, Kassem Il, Kumar A, Kessy 8M, Gebreyes W, Kazwala RRV Rajashekara G., "Antimicrobial Resistance and Genotypic Diversity of Campylobacter Isolated from Pigst Dairy, and Beef Cattle in Tanzania.". Front Microbiol. Vol. 6, el 240. 2015.
  • Chandrashekhar K, Kassem Il, Nislow CT Gangaiah D, Candetero-Rueda RAI Rajashekara G., "Transcriptome analysis of Campylobacter jejuni polyphosphate kinase (ppkl and ppk2) Virulence. Vol. 6, no. 8: 814-8. 2015.
  • Pina-Mimbela R, Madrid JA Kumar A, Torrettes JB, Rajashekara G. "Polyphosphate kinases modulate Campylobacter jejuni outer membrane constituents and alter its capacity to invade and survive in intestinal epithelial cells in vitro.". Emerg Microbes Infect Vol 4, no, e77: e77 2015.
  • Sahin O, Kassem ll. Shen Z, Lin J. Rajashekara G, and Zhang Q, "Campylobacter in Poultry: Ecology and Potential Interventions". Avian Diseases. Vol. 59, no. 2: 185-200. (2015).

2006

  • Trevor E. Swartz, Tong-Seung Tseung, Marcus A. Frederickson, Gaston Paris, Gireesh Rajashekara, Jung-Gun Kim, Mary Beth Mudgett, Gary A. Splitter, Fernando A. Goldbaum, Winslow R. Briggs, and Roberto A. Bogomolni. The Light-Activated LOV-Histidine Kinases: a New Bacterial Photoreceptor Family. Submitted for publication (Science)
  • Gireesh Rajashekara, Linda Eskra, Angie Mathison, Erik Petersen, Qiqi Yu, Jerome Harms, and Gary Splitter. Brucella: Functional Genomics and Host-pathogen interactions. Ani. Health Res. Rev. (In press)
  • Gireesh Rajashekara, David A. Glover, David O’Callaghan, Menachem Banai, and Gary A. Splitter. (2006). Attenuated bioluminescent Brucella melitensis mutants, GR019 (VirB4), GR024 (GalE), and GR026 (90-91IR) confer protection in mice. Infection and Immunity. 2006, 74:2925-36.
  • Bradley D. Anderson, Michael C. Gilson, Bryan S. Biehl, Jeremy D. Glasner, Gireesh Rajashekara, Gary A. Splitter, Nicole T. Perna. (2006). CGHScan: Finding variable regions using comparative genomic hybridization data. BMC Genomics 2006, 7:91.
  • Rajashekara, G. Glover, D.A., Krepps, M., and Splitter, G.A. 2005. Temporal analysis of pathogenic events in virulent and avirulent Brucella melitensis infections. Cellular Microbiology, 7:1459-73.
  • Gireesh Rajashekara, Michael Krepps, Linda Eskra, Angela Mathison, Alison Montgomery, Yohei Ishii, and Gary Splitter. (2005). Unraveling Brucella genomics and pathogenesis in immunocompromised IRF-1-/- mice. Am. J. Repro. Immunol. 54: 358–368.
  • Jun Zhu, Zhaohua Qiu, Christiane Wiese, Yohei Isheii, Jen Friedrichsen, Gireesh Rajashekara, and Gary A Splitter. 2005. Nuclear and mitochondrial localization signals overlap within bovine herpes virus 1 tegument protein VP22. J.Biol.Chem. 280:16038-16044
  • Rajashekara, G., Glasner, J.D., Glover, D.A., Splitter, G.A. 2004. Comparative whole-genome hybridization reveals genomic islands in Brucella species. J. Bacteriology. 186:5040-5051.
  • Seung-Hun Baek, Gireesh Rajashekara, Gary A. Splitter, and James P. Shapleigh. 2004. Denitrification genes regulate Brucella virulence in mice. J. Bacteriology. 186: 6025-6031.