Histology and Immunohistochemistry

• Tissue trimming
• Tissue processing
• Tissue embedding
• Microtomy
• Routine Hematoxylin & Eosin staining
• Routine special staining

• Frozen sectioning
• Consultation
• Ocular specialization
• Orthopedic specialization
• Advanced special staining
• Tissue MicroArray

Gram

•    Gram-positive organisms—blue to blue-violet
•    Gram negative organisms—red
•    Background—yellow

Giemsa

•    Bacteria—blue
•    Nuclei—blue
•    Cytoplasm—pale blue
•    Rickettsias—reddish purple
•    Erythrocytes—yellowish pink

Ziehl-Neelsen

•    Acid-fast bacteria—bright red
•    Other tissue elements—according to counterstain (blue or yellow)

Fites

•    Atypical mycobacteria—bright red
•    Other tissue elements—blue

Warthin Starry

•    Bacteria (spirochetes, helicobacter)—black
•    Background—pale yellow to tan

Steiner

•    Bacteria (spirochetes, legionella)—black
•    Background—pale yellow

Gimenez

•    Chlamydia—red
•    Background—green

PAS (Periodic Acid-Schiff's)

•    Simple polysaccharides, neutral mucosubstances, basement membranes, mucolipids and fungus—magenta
•    Background—according to counterstain

Alcian-Blue

•    Sulfated mucosubstances—blue
•    Background—according to counterstain

GMS (Grocott Methenamine-Silver)

•    Fungi—sharply delineated in black
•    Pneumocystis—black
•    Mucin—dark gray to black
•    Background—green
   

Wilder's Reticulum

   Reticulum fibers—black
   Background—pale pink

Gordon-Sweet

   Reticulin—black
   Background—according to counterstain

Masson's Trichrome

•    Nuclei—black
•    Cytoplasm, muscle, keratin—red
•    Collagen—according to counterstain (blue or green)

Jones'

•    Reticular fibers and basement membranes—black
•    Other tissue elements—according to counterstain

PTAH

•    Nuclei—blue
•    Cytoplasm—blue
•    Collagen—brown-pink
•    Fibrin—blue
•    Elastic fibers—purple
•    Striated muscle fibers—blue
•    Neuronal and glial cell processes—blue-purple

Verhoeff's Elastic

•    Elastic fibers—black
•    Nuclei—blue to black
•    Collagen—red
•    Other tissue elements—yellow
   

Rhodanine

• Copper—red yellow
• Nuclei—light blue

Von Kossa

• Calcium deposits—black
• Background—pink

Perl's Iron (Prussian Blue)

• Iron deposits—brilliant blue
• Background—pink

PAS (Periodic Acid-Schiff's)

•    Simple polysaccharides, neutral mucosubstances, basement membranes, mucolipids and fungus—magenta
•    Background—according to counterstain

Alcian-Blue

•    Sulfated mucosubstances—blue
•    Background—according to counterstain

Congo Red

•    Amyloid deposits—pink to red
•        Note: Amyloid will show an apple-green birefringence when polarized
     Nuclei—blue
  
   

Cresyl Echt Violet

•    Nissl substance—blue

Bielschowsky's

•    Axons—black
•    Intracellular neurofibrils—black

Luxol Fast Blue

•    Myelin sheath—blue-green
•    Background—according to counterstain
  
  PTAH
•    Nuclei—blue
•    Cytoplasm—blue
•    Collagen—brown-pink
•    Fibrin—blue
•    Elastic fibers—purple
•    Striated muscle fibers—blue
•    Neuronal and glial cell processes—blue-purple
   

Osmium

•    Lipid—black

OFG (Pituitary)

• Acidophils—orange-yellow
• Basophils—magenta-red
• Chromophobes—pale green
• RBS's—yellow

Churukian Schenk

•  Argyrophil and argentaffin cells—black
• Nuclei—orange to red
• Background—light yellow

Toluidine Blue

•  Mast cells—deep reddish purple
•  Background—faint blue