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Virus Vector Core

Director: Kristine Yoder, PhD

This core will support the individual projects of the PPG. HTLV-1 infected cells can be difficult to transfect making lentiviral vectors key to experimental success. Lentiviral vectors may be used for overexpression or shRNA reduced expression of genes of interest. CRISPR/Cas9 lentiviral vectors will be used to prevent expression of host genes or for homology directed repair when coupled with a donor DNA for homologous recombination. This research group is the first to propose CRISPR/Cas9 genome editing to disable the HTLV-1 hbz gene and prevent disease progression. Integrase defective lentiviral vectors (IDLV) are part of an unbiased method to quantify the off-target editing of CRISPR guide RNAs (gRNA). Finally, the core is developing a CRISPR/Cas9 vector with inducible, self-limiting Cas9 expression. The specific aims are:

 

Aim 1.    Provide custom retroviral vectors to the PPG labs.

Aim 2.    Design, validate, and optimize CRISPR/Cas9 vectors.

Aim 3.    Develop an inducible, self-limiting HTLV-1 CRISPR/Cas9 vector.

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