Director: Kristine Yoder, PhD
This core will support the individual projects of the PPG. HTLV-1 infected cells can be difficult to transfect making lentiviral vectors key to experimental success. Lentiviral vectors may be used for overexpression or shRNA reduced expression of genes of interest. CRISPR/Cas9 lentiviral vectors will be used to prevent expression of host genes or for homology directed repair when coupled with a donor DNA for homologous recombination. This research group is the first to propose CRISPR/Cas9 genome editing to disable the HTLV-1 hbz gene and prevent disease progression. Integrase defective lentiviral vectors (IDLV) are part of an unbiased method to quantify the off-target editing of CRISPR guide RNAs (gRNA). Finally, the core is developing a CRISPR/Cas9 vector with inducible, self-limiting Cas9 expression. The specific aims are:
Aim 1. Provide custom retroviral vectors to the PPG labs.
Aim 2. Design, validate, and optimize CRISPR/Cas9 vectors.
Aim 3. Develop an inducible, self-limiting HTLV-1 CRISPR/Cas9 vector.