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In Vivo Imaging System (IVIS) 100

Manufactured by Caliper Life Sciences

The IVIS 100 Imaging System is a highly sensitive, low light-level system optimized for in-vivo (whole, living animal) imaging. The system is composed of: 1) 1-inch ultra-sensitive CCD camera cooled with a closed cycle mechanical refrigeration unit to -105°C; 2) light-tight imaging chamber; 3) a PC computer used to operate the IVIS, collect and store images, and image analysis; 4) the Living Image® software that runs the IVIS System. The System takes very low-light level images, stores them, and displays them for subsequent analysis.

Operation of the IVIS Imaging System is simple and straightforward. Place the anesthetized animal or animals on the shelf in the imaging chamber and close the door. Collect a preliminary image for animal positioning and if necessary, adjust the field of view and focus with the AFOV and AFocus controls, in the Camera Control panel. Click Acquire in the Camera Control panel, and the system will take a picture per your settings and display the data as an overlaid image. The camera creates two images: one black-and-white (or grayscale) background photo of the mouse, and a color overlay of the emitted photon data, as shown above.

Light emitted from a luminescent source, such as luciferin, or a fluorescent source can be imaged. Cells, microorganisms, and animals that are tagged with luciferase or green fluorescent protein (and other fluorescent tags) emit low levels of detectable light. Researchers can study a range of diseases with tagged cells, including tumor cells to study cancer or bacteria cells to study infection. When researchers introduce these bioluminescently-tagged cells into an animal, they can watch;cells proliferate;with the help of a highly sensitive camera and software. The more cells there are, the brighter the light will be. By treating an animal with an experimental drug, researchers can evaluate the effect of the drug on the cancer or infection. If the treatment works, the tagged cells in the animal stop proliferating or die, leading to a corresponding change in the light emitted from the animal.


Room 0132 Veterinary Medical Center 1935 Coffey Road

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