Microcirculatory Changes in Dogs with Gastric Dilatation-Volvulus

Purpose

To evaluate the gastric serosal microcirculation in canine patients with naturally occurring GDV and to determine if buccal mucosa microcirculation or systemic parameters may serve as a surrogate, and compare gastric microcirculatory assessment to intra-operative determination of tissue viability.

Background

Many disease processes involve loss of the stomach’s blood flow which can make the disease worse and contribute to death. Evaluation of the blood flow in these diseases may improve the treatment. One such disease process is gastric dilatation volvulus. This disease occurs when the stomach fills with air and twists on its axis, trapping gas and causing great stretching of the stomach wall. This stretching leads to loss of blood flow and possible death of the tissues. This study will involve evaluation of the blood flow of the cheek and stomach in dogs with gastric dilatation volvulus. There has been no research that has evaluated the microcirculation of the stomach or cheek in this disease state. It will also measure blood flow with blood levels of lactate, a product that increases in the blood when there is decreased blood flow, and amount of tissue oxygen. While anesthetized for surgical correction of this disease process, the microcirculation of the cheek and the stomach will be evaluated with a machine that does not damage any tissue. This machine will identify red blood cells within the smallest blood vessels of the organs.

Inclusion Criteria

  • Canine patients with naturally occurring gastric dilation volvulus (GDV)

Study Design

The patients will have a Microscan performed on the stomach. This involves a handheld device that will be positioned gently on the surface of the stomach using a sterile probe cover. Prior to surgery, three-twenty second videos of the cheek microcirculation will be obtained. Once in the abdominal cavity and before the stomach is rotated, three-twenty second videos of the stomach microcirculation will be obtained. This will be repeated for the cheek at the level of the carnassial tooth. After the stomach is rotated, three-twenty second videos of the stomach microcirculation will be obtained. It will measure blood flow with blood levels of lactate, a product that increases in the blood when there is decreased blood flow, and amount of tissue oxygen. The machine will identify red blood cells within the smallest blood vessels of the organs

Client Compensation

  • Laboratory tests necessary for study data- $111 covered by the study
  • One hour of general anesthesia- $40 covered by the study

Client Cost

  • Any charges associated with the surgical procedures, postoperative care and any complications from the anesthesia or surgical procedures

Contact

Please contact Dr. Edward Cooper – 614.292.3551 or eduard [dot] cooper [at] cvm [dot] osu [dot] edu for all questions related to this clinical trial.