Research Summary

We are the first group to demonstrate inhibitory effects of calcitriol in canine cancer and normal cell lines. We demonstrated that calcitriol, EB1089, and analog V inhibit cell proliferation in SCC 2/88 cells. Calcitriol PTHrP mRNA expression and reduced stimulatory effect of TGF-β on PTHrP mRNA expression in SCC 2/88 cells. In Normal Prostate Primary Culture in Dogs: We demonstrated that VDR mRNA and 1α-OHase mRNA were present in both the epithelium and stroma of the canine prostate gland and primary cultures. VDR levels were higher in epithelial cells than in stromal cells. 1,25(OH)2D3, 25(OH)2D3, and EB1089 inhibited epithelial cell growth at 10-7M. In contrast, 1,25(OH)2D3 and EB1089 at 10-7M stimulated stromal cell growth. 1,25(OH)2D3 and its analogs may be an effective antiproliferative agent of epithelial cells in certain types of prostate cancer.

Research Funding

  1. In Vitro Effects of 1,25(OH)2D3; analog EB 1089; and Medium Chain Triglyceride on Cell Growth and Vitamin D Receptor Expression in Canine Transitional Cell Carcinoma of the Urinary Bladder.
    Principal Investigator: Dr. N Inpanbutr
    Source: The Canine Research Funds
    Co-investigators: Drs. Bill Kisselberth, CC Capen, T. Kaewsakhorn
    Collaborator: Dr. Martin Calverley, Leo Pharma Co. Denmark
  2. Effects of 1α, 25-dihydroxyvitamin D3 and Non-hypercalcemic Analogs on Arginine Esterase Activity and 1α-hydroxylase mRNA Expression in Canine Prostate Tissue Explants and Primary Cultures.
    Principal Investigator: Dr. N Inpanbutr
    Source: The Canine Research Funds
    Co-investigators: Drs. CC Capen, T. Kaewsakhorn
  3. Molecular Cloning and mRNA Expression of Equine Calbindin D-9k, Calbindin D-28k, Epithelial Calcium Channel 1, and Calcium-ATPase.
    Principal Investigator: Dr. Ramiro E. Toribio
    Co-Investigators: Drs. N Inpanbutr, TJ Rosol , CW Kohn
    Source: The Equine Research Funds
  4. Effects of 1,25-Dihydroxyvitamin D3 and Non-Hypercalcemic Analog EB 1089 on Cell Growth, Vitamin D Receptor (VDR) secretion and VDR mRNA Expression in the Canine Prostate Primary Cultures.
    Principal Investigator: Dr. N. Inpanbutr
    Co-investigators: Drs. CC Capen, T Kaewsakhorn
    Source: The Canine Research Funds

Funded Teaching Programs

  1. Faculty Collaboration Grants, Title: Introduction to Veterinary Anatomy: Source: TELR (7/2003)
  2. Web Base Program Development of Selected Surgical Procedures: Anatomy and Complete Illustrated Description of Anatomically-Challenging Procedures. Funded by: The OSU Alumni Association (9/2003)