Population genetics and mechanism of quinolone resistance in Campylobacter isolated from conventional and antibiotic free farms


Campylobacter is one of the major causes of gastroenteritis in humans resulting in more than 10,000 hospitalization and treatment cost exceeding 1.7 million (Mead et al. 1999).  It is a self limiting disease and does not require antimicrobial treatment. But in rare cases where there is systemic involvement and in immunocompromised individuals, macrolides and fluoroquinolones are the drug of choices. Understanding the population genetics is important in identifying the sources and vehicles of contaminations for better understanding of the epidemiology and safeguarding the public health.


Isolates of Campylobacter from two different production systems (conventional and antibiotic free) and different geographic locations are included.

Goals:

                      Sequence

  •   To evaluate the population dynamics and genetic diversity/clonality of Campylobacter. Due to the instability of the genome of Campylobacter, we focused on the seven housekeeping loci (aspA, glnA, gltA, glyA, pgm, tkt, and uncA) to study the genetic diversity using multilocus sequence typing (MLST) method.

                                         

           sequence                              

 

  • To investigate variation in the quinolone resistance determining region (QRDR) of the gyrA and parC genes on selected quinolone resistance and susceptible Campylobacter strains.             

 

 

 

  

Personnel:

Daniel A. Tadesse (PhD student)
Wondwossen A. Gebreyes (Principle Investigator)

Other Collaborators:

Morgan Morrow (North Carolina State University)
Peter Bahnson (University of Wisconsin at Madison)
Julie Funk (Michigan State University)

Funding:

USDA grant 2002-51110-01508