Phenotypic and genotypic characterization of multi-drug resistant Salmonella Heidelberg isolated from human and animals
Salmonella serovar Heidelberg is one of the top ten serovars causing foodborne infection in humans. In the present study, we investigate antimicrobial drug resistant patterns and molecular characterizations for 99 Salmonella Heidelberg isolated from human, pig, and turkey.
The common resistant patterns found among Salmonella strain isolated in human and pigs show various multi-drug resistant patterns.
The genotypic characterization by Pulse Field Gel Electrophoresis (PFGE) of multi-drug resistance Salmonella Heidelberg is also revealing clonality of MDR strains. Understanding the diversity of MDR Salmonella strains, appreciation of potential transfer mechanisms among bacterial pathogens is critical for an epidemiological model of Salmonella infection in various host species.
Our specific study objectives are the following:
- To identify the predominant phenotypes of Salmonella Heidelberg among different host species using antimicrobial resistance patterns.
- To determine the genotypic diversity of these isolates using Pulse Field Gel Electrophoresis DNA fingerprinting method
- To identify the resistant genes and its potential of MDR gene transfer among and between bacterial species