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Phenotypic and genotypic characterization of multi-drug resistant Salmonella Heidelberg isolated from human and animals

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<em><img align="right" alt="Antibiotic susceptability plate" height="200" hspace="5" src="/assets/images/prevMed/GebreyesLaboratory/Research/Biocide/Plt-3-v1.JPG" vspace="5" width="205" />Salmonella</em> serovar <em>Heidelberg </em>is one of the top ten serovars causing foodborne infection in humans. In the present study, we investigate antimicrobial drug resistant patterns and molecular characterizations for 99 <em>Salmonella Heidelberg</em> isolated from human, pig, and turkey.<br />
The common resistant patterns found among <em>Salmonella </em>strain isolated in human and pigs show various multi-drug resistant patterns.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<img align="left" alt="PFGE" height="258" hspace="5" src="/assets/images/prevMed/Gebreyes%20Laboratory/Picture2.png" style="width: 297px; height: 258px" vspace="5" width="319" />The genotypic characterization by Pulse Field Gel Electrophoresis (PFGE) of multi-drug resistance <em>Salmonella Heidelberg</em> is also revealing clonality of MDR strains. Understanding the diversity of MDR <em>Salmonella</em> strains, appreciation of potential transfer mechanisms among bacterial pathogens is critical for an epidemiological model of <em>Salmonella</em> infection in various host species.
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Our specific study objectives are the following:
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<li>To identify the predominant phenotypes of <em>Salmonella Heidelberg</em> among different host species using antimicrobial resistance patterns. </li>
<li>To determine the genotypic diversity of these isolates using Pulse Field Gel Electrophoresis DNA fingerprinting method </li>
<li>To identify the resistant genes and its potential of MDR gene transfer among and between bacterial species</li>
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<h3>IDMEL Personnel:</h3>
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<a href="/4034.htm">Prapas Patchanee</a>&nbsp;(PhD Student)<br />
<a href="/2519.htm">Wondwossen Gebreyes</a>&nbsp;(Principle Investigator)
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