Raw milk samples were collected from 15 different farms in the State of Paraíba of Northeastern Brazil. The farms are representative of the entire State. A total of 552 raw milk samples were collected from 138 apparently healthy cows.
Staphylococcus isolation was done using conventional methods. Agar diffusion method was used to phenotypically characterize all the isolates for 11 antimicrobial agents. Multiplex PCR was used to genotypically characterize the isolates by SCCmec-type (I-IV) and mecA gene amplification.
Staphylococcus isolates were common in milk. This could impose significant food safety hazard to consumers. Methicillin resistance is present in whole milk. Northeastern Brazil is the poorest region of Brazil and no studies have been conducted in antimicrobial resistance in any type of pathogens from this region.
Narry Tiao (Master's Student)
Wondwossen Gebreyes (Principle Investigator)
Lauro Santos (Professor, Universidade Federal da Paraiba)
Celso Bruno (Professor, Universidade Federal da Paraiba)