Molecular mechanisms of resistance in Acinetobacter baumannii


efflux pumpAcintebacter baumannii is one of the most common emerging healthcare-associated human pathogens that currently cause numerous hospital outbreaks, and increasingly reported as a cause of human infection, particularly ventilator-associated pneumonia, bloodstream infections, urinary tract infections, and wound infections. This organism is increasingly showing multi-drug resistance (MDR) to various classes of antimicrobials and disinfectants. Using various molecular biology techniques, such as recombinant DNA technology, DNA sequencing and computer-aided DNA sequence analysis, site-directed mutagenesis, protein analysis, and protein structural studies we are trying to elucidate:

  • To identify multidrug efflux pumps involved in mediating resistance against antibiotic, bile and antimicrobial peptides. Our emphasis would be to delineate the role of secondary transporters in biocide resistance.
  • To perform structure function analysis of the secondary transporters.
  • To characterize the regulatory network for the secondary transporters.

          
Studying the role of secondary transporters in bacteria will unravel the multiple facets which will enable us to design novel prevention strategies.

 

IDMEL personnel:

Govindan Rajamohan (Postdoctoral Researcher)
Srinivasan Vijaya Bharathi (Postdoctoral Researcher)
Wondwossen A. Gebreyes (Principle Investigator) 

Collaborators:

Dr. Mario Marcon (Director of Clinical Microbiology, Nationwide Children's Hospital)
Dr. Preeti Pancholi (Director of Clinical Microbiology, OSUMC)
Dr. Kurt Stevenson (Associate Professor, Infectious Diseases)

Source of Funding:

The Ohio State University