Genotypic characterization by SCCmec-type and clonality of clinical Staphylococci isolates from a veterinary hospital

Staphylococci, especially Methicillin-resistant Staphylococcus aureus (MRSA), cause a large proportion of the nosocomial infections in human hospitals globally.  However, there are few studies on the status of MRSA isolated from veterinary sources. MRSA is hypothesized to be of significant concern in companion animals because of their close association with their human owners. This potential zoonotic, or reverse zoonotic, transmission could be a major public health concern.

The purpose of this study was to genotypically characterize Staphylococci clinical isolates obtained between Feb 2007-Feb 2008 from companion animals from The Ohio State University Veterinary Medical Center.  In addition, the clonality of the isolates was investigated.

Isolates from the veterinary medical center were genotypically characterized by Staphylococcal Chromosomal Cassette (SCCmec) typing (I-IV) and mecA characterization using multiplex Polymerase Chain Reaction (PCR).  The presence of mecA gene labels the isolate as a MRSA.  A modified version of Oliveira and Lancastre (2002) protocol was used for the multiplex PCR.  The isolates were also analyzed by Pulse-Field Gel Electrophoresis (PFGE) for clonality.

staph PCR

IDMEL Personnel

Narry Tiao (Master's Student)
Wondwossen Gebreyes (Principle Investigator)

Funding

The Ohio State University