The IDMEL is located in Room no. 367 and 371 of third floor of Veterinary Medical Academic Building (VMAB), College of Veterinary Medicine, The Ohio State University. The laboratory is equipped with various instruments to do clinical, field (on-farm) as well as molecular microbiological and epidemiological investigations that enable to successfully address foodborne and nosocomial pathogen concerns. The IDMEDL is primarily engaged in conducting research in various fundamental issues on Multidrug resistant foodborne and nosocomial bacterial pathogens. We have the following gravitational research facilities, besides the other centralized access to various modern and advanced instruments.
Bacterial culture and phenotyping: The laboratory is well-equipped with all the necessary instrumentation to isolate and identify various foodborne and nosocomial pathogens. In addition, phenotyping methods including serogrouping of Salmonella and antimicrobial susceptibility testing of various pathogens are routinely carried out using various approaches including Kirby-Bauer disc diffusion, agar dilution and broth microdilution approaches. For details of tests, fee schedules and other information, please visit the services page.
Gene detection and amplification: We perform traditional PCR and real time PCR for the detection and quantification of the various genes. We have the facilities to execute the gene detection and amplification procedures such as Air clean 600 PCR workstation, Stratagene Mx3000P and Mx3005P real time PCR machines connected with dedicated individual computers, MJ and Eppendrof Thermocyclers, Bio-Rad Gel doc 2000 system connected with dedicated computer having Quantity one software, multiple DNA electrophoresis gel-casting units etc.
Molecular subtyping (PFGE, AFLP, MLST and others): We carry out molecular subtyping mainly by using Biorad CHEF-DR III pulsed field gel electrophoresis (PFGE) system. The gel image from PFGE gel will be captured using Bio-Rad Gel doc 2000 workstation. The resulting PFGE, AFLP patterns, analyzed by using the BioNumerics® software package. We execute Amplified Fragment Length Polymorphism (AFLP) and Multi locus Sequence Typing (MLST) by using Beckman coulter CEQ 8000 Genetic analyser.
DNA sequencing: We carry out DNA sequencing by using Beckman coulter CEQ 8000 Genetic analyser attached with dedicated IBM server / workstation. This system is an 8-capillary electorphoresis based platform capable of running up to 12 different protocols simultaneously for sequencing, SNP and AFLP analyses.
Molecular and Protein analysis: We perform both molecular and protein analysis by using various tools such as ; Air clean 600 PCR workstation, Stratagene Mx3000P and Mx3005P real time PCR machines connected with dedicated individual computers, MJ and Eppendrof Thermocyclers, Biorad Gel doc 2000 system connected with dedicated computer having quantity one software, multiple DNA and protein electrophoresis gel-casting units, Biorad western apparatus, Genesys 20 spectrophotometer, biomate 3 Spectrophotometer, biological safety cabinet, and Fischer Hamilton chemical hood, Forma scientific reach-in incubator, Isotemp laboratory refrigerator etc.
Other Microbiological activities: We have dedicated room equipped with necessary accessories such as class II Biosafety hood, chemicals, glass wares, plastic wares etc to aseptically prepare various media needed for routine microbiological research, bacterial culture and recombinant bacterial culture propagation.
In addition to Room no. 367 and 371 in third floor of Veterinary Medical Academic Building (VMAB), we have Room no. A107 in Sisson Hall which is currently shared with another PI. The room is well equipped with required facilities and instrumentation and is being used exclusively for field sample processing, isolation and identification of food borne pathogens.