College of Veterinary Medicine

Affymetrix GeneChip Expression Analysis Common Room Lab (Room 365 VMAB)

The Affymetrix node consists of all equipment and software to process the arrays and analyze our equine gene expression microarray data, as well as any other Affymetrix GeneChip. The node functions under the direction of Dr Bertone and is housed 30 ft from her research laboratory. Affymetrix arrays are scanned at the Microarray Core Facilities at The Ohio State University James Cancer Institute or Davis Heart and Lung Institute, of which Dr Bertone is member. The node is comprised of the Affymetrix Genechip Fluidics Station 400, the Affymetrix GeneChip Hybridization Oven 640, and a Dell Optiplex GX150 which uses the Affymetrix Microarray Suite 5.0 and Data Mining Tool software. The GeneChip probe array Fluidics Station 400 contains four modules; each module can hold one GeneChip probe array cartridge and can be run independently of the others. The Fluidics Station hybridizes, washes, and stains the GeneChip probe and tags. The GeneChip Hybridization Oven 640 is capable of hybridizing up to 64 probe array cartridges with precision temperature control. The Affymetrix Microarray Suite 5.0 controls the fluidics station, analyzes experimental data, generates report summaries, etc. Further bioinformatics support is available on an hourly basis from the Core Facilities.