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The Comparative Orthopedic Molecular Medicine Suite is room 325 of the Veterinary Academic Medical Building (VMAB). The VMAB is located at The Ohio State University’s Veterinary Campus and is a > 100,000 sq. ft. facility dedicated to research laboratories on the top 2 of 4 floors. The Comparative Orthopedic Molecular Medicine Suite is 1069 sq.ft of laboratory space with bench tops, internet-connected workstations, shelving and cabinets contained in modules B, C and D.
Room 325B is 550 sq. ft. dedicated to DNA processing and gene expression analysis and contains two laminar flow hoods, a –80C freezer, an Iso Temp – 20C freezer, refrigerator, Applied BioSystems Taqman 7000 Sequence Detection System (RT-PCR analyzer) and dedicated PC computer with Primer Express software, refrigerated Micro centrifuge, (2) MJ minicycler PCR Centrifuge engines, a Sorvall RC-5C Microcomputer Controlled Super speed Refrigerated Centrifuge with changeable rotors, New Brunswick Incubator/Shaker, Eppendorf Electroporator 2510, electrophoresis gel-casting units, pH meter, analytical balance, water and dry baths, pipetters, supplies, glassware and other accessories necessary for molecular biology applications. Also in 325B, a central area houses a BioMate3 Spectrophotometer, Zylux Tube Luminometer, Kodak GelDoc Photography digital imaging system, and ultra Microplate reader served by a Dell 1.7 Gb processor. An iMac (1 Gb) computer with accessories including a digital still/video cam recorder, 6-card reader, and CD burning capabilities serves this section. This card reader serves as the computer interface for the digital image capture systems on the GelDoc, Olympus CK40 Inverted and CX41 Compound Microscope and Canon Elura 20 MC Video chip/digital camera.
Room 325C is 450 sq. ft. dedicated to cell/tissue culture and vector propagation and contains two laminar flow hoods, one that operates at BSL2-level containment. The module contains (2) ThermoForma water jacketed forced air, reach-in CO2 incubators, water bath, pipetters, refrigerator, Maytag dishwasher, Barnstead thermolyne autoclave, tissue homogenizer, liquid nitrogen storage tank, Olympus CK40 inverted florescent microscope with viewing screen, Olympus DP12 digital camera and Olympus P330N color printer. A power G3 computer serves this section.
Room 325D is 100 sq. ft. dedicated to imaging and processing and houses an Olympus CX41 phase-compound microscope with a camera and monitor, and a Zeiss stereomicroscope. This area is the processing center for the other modules and houses the common color printer, laser printer, and scanner. An iMac computer serves this module. A second laminar flow chemical hood is dedicated to decalcification of gross bone/cartilage specimens. Additionally, Dr Bertone shares a 250 sq. ft. radioisotope laboratory equipped with two laminar flow hoods, CO2 jacket incubator, and scintillation counter for cell/tissue culture metabolic studies. Access is also available to all common equipment in the common laboratories on the 3rd and 4th floors of the Veterinary Medical Academic Building. These facilities include additional high-speed and ultra speed centrifuges, scintillation counters, sonicator, cryostat, controlled temperature rooms, dark room, autoclave room, ice maker, glassware facility, radiographic film processor, and fluorescence gel documentation equipment.
Laboratory for Gait Analysis and Applied Research
The Laboratory for Gait Analysis and Applied Research is Room 1338 of the Veterinary Medical Center and is directly adjacent to the in ground force plate.
This 425 sq.ft. laboratory contains an animal holding and working area separated from the equipment and laboratory area by containment rails. This laboratory is designed for direct evaluation of live research animals to permit short procedures using hospital equipment, such as fluoroscopy, ultrasound and quantitative computer tomography on research animals (rabbits, cats, sheep, dogs, horse, calves etc.) in a designated area. This section has a central drain, rubber floor, containment rails, hoses and cabinets. The laboratory section contains 32 sq.ft of counter/working bench space, an undercounter refrigerator, 110 sq. ft. feet of locked cabinets, 6.8 sq. ft. of drawer storage space, two internet access ports, a desk and phone, and a sink.
The entrance to room 1338 permits visualization of the in ground Kistler force plate (3ft x 2 ft) in the 60 ft. hallway directly outside this laboratory. All connections to the forceplate feed into room 1338 which houses the designated gait analysis PC computer. A second computer, a portable G3 PowerMac Powerbook, services this laboratory.
Affymetrix GeneChip Expression Analysis Common Room Lab (Room 365 VMAB)
The Affymetrix node consists of all equipment and software to process the arrays and analyze our equine gene expression microarray data, as well as any other Affymetrix GeneChip. The node functions under the direction of Dr Bertone and is housed 30 ft from her research laboratory. Affymetrix arrays are scanned at the Microarray Core Facilities at The Ohio State University James Cancer Institute or Davis Heart and Lung Institute, of which Dr Bertone is a member. The node is comprised of the Affymetrix Genechip Fluidics Station 400, the Affymetrix GeneChip Hybridization Oven 640, and a Dell Optiplex GX150 which uses the Affymetrix Microarray Suite 5.0 and Data Mining Tool software. The GeneChip probe array Fluidics Station 400 contains four modules; each module can hold one GeneChip probe array cartridge and can be run independently of the others. The Fluidics Station hybridizes, washes, and stains the GeneChip probe and tags. The GeneChip Hybridization Oven 640 is capable of hybridizing up to 64 probe array cartridges with precision temperature control. The Affymetrix Microarray Suite 5.0 controls the fluidics station, analyzes experimental data, generates report summaries, etc. Further bioinformatics support is available on an hourly basis from the Core Facilities.