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We recently purchased the PRECISION Image Analysis Toolbox. This sophisticated software package will allow us to take our digital slide scanning to a whole new level by offering image analysis options. We are able to perform relative quantification of histochemical and immunohistochemical stains. Contact the Image Analysis Specialist (haramia [dot] 2 [at] osu [dot] edu) to set up a consultation and obtain pricing.
Module includes brightfield color deconvlution and colocalization tools. Separate multiple stains in brighfield whole slide images. Once separated, intensity, stain area, and colocalization of each stain is calculated.
Whole Cell Quantification
Includes nuclear, membrane, and cytoplasmic analysis tools. Count stained cells or nuclei and measure stain intensity within nuclei, membranes, or cytoplasm.
Includes Microvessel analysis and rare event detection. Measure Microvessel density (MVD), vessel area, vessel perimeter, and measure lumen. Find rare stained cells on a slide, locate objects using x-y coordinates on slide, sort by size or intensity.
Application of Algorithms (http://www.aperio.com/lifescience/precision/cases):
** Positive Pixel Count is offered free to everyone and the user guide is available here (insert link to pdf)
Submit a request (link to https://cpmpsr.osumc.edu/Forms/Public/Login.aspx?ReturnUrl=%2fForms%2fMain.aspx)