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Real-time PCR: Lightcycler 480 and 1.2
The Roche LightCycler 1.2 is a first generation real-time PCR instrument. The instrument consists of an air-heated/cooled chamber and fluorimeter. Real-time detection is by SybrGreen or specific fluorescent-labeled hybridization probes (supports FRET, TaqMan and molecular beacon probe technologies). Cycling times are rapid (20-60 minutes/run) and the software offers the capability to perform standard and multiplex PCR product analysis, quantitation and mutation analysis. Sample carousel can accommodate up to 32 samples per run.
The LightCycler® 480 Real-Time PCR System is a novel multiwell-plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids and genotyping. Building on the benefits of Roche's capillary-based LightCycler® 1.5 and 2.0 Systems, it goes one step further in offering enhanced throughput, compatibility with automation equipment and maximum flexibility regarding hard-and software.
LightCycler® 480 Real-Time PCR System Features:
Speed & Accuracy
- Novel thermal cycling and data capture technologies to achieve outstanding temperature homogeneity and assay reproducibility
- Analysis of true raw data, without the need to use passive reference dyes or normalization plates to get accurate results
Versatility & Convenience
- 96-well plate format
- Compatible with numerous assay formats and detection dyes
- Intuitive, user-friendly Roche software interface, including novel step-saving short cuts for high-throughput sample and data handling.
- Store and flexibly re-analyze the same raw data subsequently with different algorithms, depending on your needs
- Extensive analysis capabilities of the modular LightCycler® 480 Software, with high-end algorithms that ensure accuracy and reproducibility
Sign-in at use, appointment generally not necessary. Limited 24-hour access laboratory (key card entry).
New user education
Dr. Kate Hayes supervises the use of the LightCycler 480; she should be contacted prior to initial use. This instrument is supported with technical expertise to help with training for the primary user.
- Quantification using real-time PCR technology: applications and limitations. Klein, D. Trends in Molecular Medicine 8:257-260, 2002.
- A new mathematical model for relative quantification in real-time RT-PCR. Pfaffl, M.W. Nucleic Acids Research 29:2000-2007, 2001.
- Real-time PCR in virology. Mackay, I.M., Arden, K.E. and Nitsche, A. Nucleic Acids Research 30:1292-1305, 2002.
- LightCycler qPCR optimization for low copy number target DNA. Teo, I.A., Choi, J.W., Morlese, J., Taylor, G. and Shaunak, S. Journal of Immunological Methods 270:119-133, 2002.
Real-time PCR links
Primer/Probe Database Links