Real-time PCR: Lightcycler 480 and 1.2

LightCycler 1.2  

Description

Real-time PCRThe Roche LightCycler 1.2 is a first generation real-time PCR instrument. The instrument consists of an air-heated/cooled chamber and fluorimeter. Real-time detection is by SybrGreen or specific fluorescent-labeled hybridization probes (supports FRET, TaqMan and molecular beacon probe technologies). Cycling times are rapid (20-60 minutes/run) and the software offers the capability to perform standard and multiplex PCR product analysis, quantitation and mutation analysis. Sample carousel can accommodate up to 32 samples per run.

LightCycler 480

Description

The LightCycler® 480 Real-Time PCR System is a novel multiwell-plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids and genotyping. Building on the benefits of Roche's capillary-based LightCycler® 1.5 and 2.0 Systems, it goes one step further in offering enhanced throughput, compatibility with automation equipment and maximum flexibility regarding hard-and software.

LightCycler® 480 Real-Time PCR System Features:

Speed & Accuracy

  • Novel thermal cycling and data capture technologies to achieve outstanding temperature homogeneity and assay reproducibility
  • Analysis of true raw data, without the need to use passive reference dyes or normalization plates to get accurate results

Versatility & Convenience

  • 96-well plate format
  • Compatible with numerous assay formats and detection dyes
  • Intuitive, user-friendly Roche software interface, including novel step-saving short cuts for high-throughput sample and data handling.
  • Store and flexibly re-analyze the same raw data subsequently with different algorithms, depending on your needs
  • Extensive analysis capabilities of the modular LightCycler® 480 Software, with high-end algorithms that ensure accuracy and reproducibility

Location

Room 219
Goss Laboratory

Contact & scheduling

Kate Hayes-Ozello
(614) 292-8670
e-mail

Arumugam [dot] 1 [at] osu [dot] edu (Prakash Arumugam)
(614) 688-3222

Sign-in at use, appointment generally not necessary. Limited 24-hour access laboratory (key card entry).

Learn more

New user education

Dr. Kate Hayes supervises the use of the LightCycler 480; she should be contacted prior to initial use. This instrument is supported with technical expertise to help with training for the primary user.

  • Quantification using real-time PCR technology: applications and limitations. Klein, D. Trends in Molecular Medicine 8:257-260, 2002.
  • A new mathematical model for relative quantification in real-time RT-PCR. Pfaffl, M.W. Nucleic Acids Research 29:2000-2007, 2001.
  • Real-time PCR in virology. Mackay, I.M., Arden, K.E. and Nitsche, A. Nucleic Acids Research 30:1292-1305, 2002.
  • LightCycler qPCR optimization for low copy number target DNA. Teo, I.A., Choi, J.W., Morlese, J., Taylor, G. and Shaunak, S. Journal of Immunological Methods 270:119-133, 2002.

Real-time PCR links

Primer/Probe Database Links