Confocal Microscopy

Description

LeicaThe confocal system is a Leica TCS SP2 AOBS Confocal Laser Scanning Microscope. The system is equipped with 4 lasers (Red HeNe 633nm, Green HeNe 543nm, Argon 458/476/488/496/514 nm, Argon UV) with 3 PMTs and is capable of collecting up to 3 colors simultaneously and up to 8 colors with each image. Common uses include quantitative confocal image analysis, 3D reconstruction, FRAP, and FRET. Scaning resolution is up to 4096 x 4096 pixels. Software includes 3D reconstruction with rendering, multi-color analysis, FRAP analysis, FRET analysis, animation, sterio imaging, single layer projection and quantitative analysis, time lapse collection, ratio quantification, and co-localization analysis of 2 or 3 colors.

Requirement for Access to the Confocal Microscope

  • Complete a short course (~one hour) on the use and operation of the microscope
  • Have Buck ID card  access to Rm 101A Goss Laboratories
  • Have a password for access to system
  • Schedule time by appointment with Dr. Lawerence Mathes

Location

Room 111 Goss Laboratories
1925 Coffey Rd.

Contact Person

mathes [dot] 2 [at] osu [dot] edu

Fees

Fee Schedule (per hour)

ClientFee for Service
OSU: Non-UV $25
OSU: UV $35.00
Non-OSU $125.00