Collection and Processing
Sample Collection
Serum
Collect at least 5 ml whole blood in a red top "clot tube", centrifuge and freeze serum in 0.5ml aliquots.
Plasma
Collect at least 4-6 ml whole blood in EDTA tube(s). Centrifuge and collect plasma. Centrifuge plasma a second time, collect and freeze in 0.5 ml aliquots.
Urine
Collect 2-3 ml urine by free catch or cystocentesis and freeze in 0.5 ml aliquots.
Whole Blood
Collect 22 ml whole blood for 2 PaxGene RNA tubes (2.5 ml. each) and 2 PaxGene DNA tubes (8.5 ml. each).
Tissue Processing
Begin tissue processing within 5 minutes after the tissue has been resected. Process tumor and normal tissue for storage 3 different ways:
- Flash frozen in 100 mg aliquots
- Embedded in OCT medium
- Fixed in formalin
